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Image Search Results
Journal: Redox Biology
Article Title: Compartmentalized regulation of NAD + by Di (2-ethyl-hexyl) phthalate induces DNA damage in placental trophoblast
doi: 10.1016/j.redox.2022.102414
Figure Lengend Snippet: Summary diagram. DEHP decreases the overall NAD + level of the placenta and reduces the NAD + level in the placental nucleus by participating in the regulation of NAD + regionalization. By destroying the placental mitochondrial structure, reducing the number of mitochondria, hindering the way of electron transfer in the respiratory chain, DEHP reduces ATP production. Under the combined action of NAD + and ATP depletion, inhibition of PARP1 activity leads to the accumulation of DNA damage and ultimately to the reduction of the placental trophoblast area. The partially damaging effects of DEHP on DNA and mitochondrial structures can be alleviated by moderate supplementation of NAD + precursor NAM.
Article Snippet: The enzymatic activities of complex I (NADH dehydrogenase), complex II (succinate-coenzyme Q reductase), complex III (CoQ-cytochrome C reductase), complex IV (Cytochrome C oxidase) and complex V (F 1 F 0 -ATP synthase) were assayed using
Techniques: Inhibition, Activity Assay